The dense 3D structure of in vitro tumor models poses significant challenges for traditional imaging. To address this, our team at RPI developed an optical coherence tomography (OCT) method combined with Imaris software for label-free, nondestructive measurement of aggregate morphology (e.g., volume, sphericity) and live cell counts. This approach allows for longitudinal quantitative tracking of 3D morphology, cell number, and regional cell density within the same tumor model during model maturation and in response to treatment. Using this workflow, we observed that different biofabrication methods produce aggregates with distinct morphologies and that drug resistance increases with aggregate size (IC50: 3D > 2D). Region-specific viability measurements further highlight a drug’s penetration efficacy within solid tumors.

Lead PI: Dr. David Corr