Three-dimensional (3D) microenvironments are crucial for accurately replicating the cellular conditions within solid tumors, including high cell density, 3D context, direct cell-cell interactions, and oxygen and nutrient gradients. These critical features can be effectively reproduced in vitro with 3D tumor models; however, the size and shape of the models are vital for establishing appropriate gradients. Our team at RPI has developed a laser direct write (LDW) bioprinting method that provides precise control over the size and shape of dense cellular aggregates, such as tumor spheroids and embryoid bodies, without relying on exogenous factors like Matrigel. This method also enables the creation of heterogeneous tumor models with spatially defined compositions, enhancing the physiological relevance of in vitro tumor studies.

Lead PI: Dr. David Corr